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ACTIVIDAD BIOLÓGICA DE EXTRACTOS EN ACETATO DE ETILO DE LOS HONGOS FUSARIUM CAMPTOCERAS WOLLENW Y REINKING Y ASPERGILLUS FLOCCULOSUS FRISVAD Y SAMSON, AISLADOS DE AMBIENTES MARINOS Boletín de Investigaciones
Acosta,Mercedes; Guevara,Miguel; Crescente,Óscar.
Los hongos marinos se han convertido en una fuente importante de metabolitos farmacológicamente activos. Los extractos en acetato de etilo de los hongos marinos Fusarium camptoceras y Aspergillus flocculosus fueron evaluados para determinar su actividad antibacteriana, antifúngica, fototóxica y tóxica contra Artemia franciscana. Los hongos fueron cultivados bajo condiciones estáticas en agar CYA durante 14 días a 27 °C, y luego extraídos en acetato de etilo por siete días. Los extractos de ambas especies fúngicas mostraron una importante actividad antibacteriana contra bacterias Gram positivas y Gram negativas, con halos de inhibición que alcanzaron los 30 mm de diámetro. La actividad fototóxica estuvo ausente y sólo se observó actividad antifúngica en el...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusarium; Aspergillus; Bioactividad; Hongos marinos.
Ano: 2011 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612011000100002
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Actividad biológica de extractos en acetato de etilo de los hongos Fusarium camptoceras wollenw y Reinking y Aspergillus flocculosus Frisvard y Samson, aislados de ambientes marinos OceanDocs
Acosta, Mercedes; Guevara, Miguel; Crescente, Óscar.
Los hongos marinos se han convertido en una fuente importante de metabolitos farmacológicamente activos. Los extractos en acetato de etilo de los hongos Fusarium camptoceras y Aspergillus flocculosus fueron evaluados para determinar su actividad antibacteriana, antifúngica, fototóxica y tóxica contra Artemia franciscana. Los hongos fueron cultivados bajo condiciones estáticas en agar CYA durante 14 días a 27 °C, y luego extraídos en acetato de etilo por siete días. Los extractos de ambas especies fúngicas mostraron una importante actividad antibacteriana contra bacterias Gram positivas y Gram negativas, con halos de inhibición que alcanzaron los 30 mm de diámetro. La actividad fototóxica estuvo ausente y sólo se observó actividad antifúngica en el extracto...
Tipo: Journal Contribution Palavras-chave: Active; Fusarium; Aspergillus.
Ano: 2011 URL: http://hdl.handle.net/1834/4380
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Anthropization Effects on the Filamentous Fungal Community of the Brazilian Catimbau National Park Rev. Bras. Ciênc. Solo
Cruz,Roberta; Ramos,Sérgio Murilo Sousa; Fonseca,Julyanna Cordoville; Motta,Cristina Maria de Souza; Moreira,Keila Aparecida.
ABSTRACT The Caatinga biome features an exclusive endemic biodiversity, and is characterized by the presence of xerophytic, deciduous vegetation, high temperatures, and low rainfall. This important park has undergone anthropization, especially through extraction of firewood and timber and growing plants for raising goats. The objectives of this study were to compare the communities of filamentous fungi present in the preserved area and in the anthropized soil of the Catimbau National Park in Buíque, PE, Brazil, and to evaluate the impacts of anthropization on such communities. A total of 12 collections of soil samples were made, six in the preserved area and six in the anthropic area, and the physicochemical properties of the soil samples were analyzed....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caatinga; Diversity; Aspergillus; Gongronella; Penicillium.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100419
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Antifungal activities of thiosemicarbazones and semicarbazones against mycotoxigenic fungi Ciência e Agrotecnologia
Paiva,Rojane de Oliveira; Kneipp,Lucimar Ferreira; Goular,Carla Marins; Albuquerque,Mariana Almeida; Echevarria,Aurea.
Mycotoxigenic fungi can compromise the quality of food, exposing human and animal health at risk. The antifungal activity of eight thiosemicarbazones (1-8) and nine semicarbazones (9-17) was evaluated against Aspergillus flavus, A. nomius, A. ochraceus, A. parasiticus and Fusarium verticillioides. Thiosemicarbazones had MIC values of 125-500 µg/ml. The thiosemicarbazones 1 and 2 exerted fungistatic activity against Aspergillus spp., and thiosemicarbazone 2 exerted fungicidal activity against F. verticillioides. Compound 2 showed an iron chelating effect of 63%. The ergosterol content of A. parasiticus had a decrease of 28 and 71% for the 31.2 and 62.5 µg/ml concentrations of thiosemicarbazone 2 compared to the control. The obtained results of antifungal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus; Fusarium; Mycotoxicology; Thiosemicarbazones.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000600001
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Antioxidant Activities of Chicken Egg White Hydrolysates Obtained by New Purified Protease of Aspergillus avenaceus URM 6706 BABT
Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Oliveira,João Tiago Correia; Medeiros,Erika Valente; Souza-Motta,Cristina Maria de; Moreira,Keila Aparecida.
Abstract Protein hydrolysates originating from egg white have already been reported to be bioactive and, among their biological activities, possess the antioxidant property that protects the body from early ageing and diseases linked to oxidation. Therefore, the objective of this work was to evaluate the antioxidant activity of hydrolysates obtained by the hydrolysis of egg white from hen poultry. The protease produced by Aspergillus avenaceus URM 6706 was purified and subsequently applied to hydrolysate the egg white, and the degree of hydrolysis was verified during the protease exposure time (4-24 h). The hydrolysis was intensified over time of exposure to the protease. It was possible to detect the antioxidant activities of eliminating the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus; Purification; ABTS; +; DPPH; Chelators.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100508
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Aspectos relacionados à presença de fungos toxigênicos em uvas e ocratoxina A em vinhos Ciência Rural
Welke,Juliane Elisa; Hoeltz,Michele; Noll,Isa Beatriz.
A colonização dos Aspergillus da secção Nigri nas uvas durante o cultivo é a principal fonte de ocratoxina A (OTA) nos vinhos. A. carbonarius e A. niger são os principais produtores desta micotoxina em uvas e são fungos oportunistas que, se desenvolvem, principalmente, nas bagas danificadas durante seu amadurecimento. A produção de OTA em uvas é influenciada pelas condições climáticas e áreas geográficas, bem como pela variedade de uva, pelo sistema de cultivo e pelos danos causados nas uvas por insetos, infecção fúngica ou excesso de irrigação e chuva. As medidas para o controle de fungos toxigênicos devem considerar esses pontos críticos de controle. A OTA presente nas uvas é transferida para o vinho durante o processo de vinificação, sendo que um...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ocratoxina A; Uva; Vinho; Aspergillus.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000800049
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Assay of antioxidant potential of two Aspergillus isolates by different methods under various physio-chemical conditions BJM
Arora,Daljit Singh; Chandra,Priyanka.
The objective of this work was to screen fungi isolated from soil of different areas of Punjab, India for antioxidant activity by dot blot assay and around 45% of fungal isolates demonstrated antioxidant potential. Two selected strains of Aspergillus spp (Aspergillus PR78 and Aspergillus PR66) showing quantitatively best antioxidant activity by DPPH assay were further tested for their reducing power, ferrous ion and nitric oxide ion scavenging activity, FRAP assay and total phenolic content. Different physio-chemical parameters were optimized for enhancement of the activity. This revealed stationary culture grown for 10 days at 25ºC at pH 7 to be the best for antioxidant activity. Sucrose in the medium as carbon source resulted in highest antioxidant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant potential; Aspergillus; Dot blot assay; DPPH assay; FRAP assay; Reducing power; Soil fungi.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300029
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Atividade celulolítica de fungos isolados de bagaço de cana-de-açúcar e madeira em decomposição PAB
Basso,Thalita Peixoto; Gallo,Cláudio Rosa; Basso,Luiz Carlos.
O objetivo deste trabalho foi identificar isolados de fungos a partir de bagaço de cana-de-açúcar e madeira em decomposição e avaliar a sua atividade celulolítica em bagaço de cana. Cinco isolados foram avaliados, tendo-se como referências os fungos Trichoderma reesei QM9414 e T. reesei RUT C30. A atividade celulolítica foi estimada pela capacidade hidrolítica do extrato enzimático dos fungos cultivados em bagaço de cana sobre os substratos papel de filtro (atividade celulolítica total) e carboximetilcelulose sódica (atividade da endoglucanase). Os isolados foram identificados pela análise molecular da região 26S rDNA. Os gêneros Paecilomyces, Aspergillus, Acremonium/Penicillium e Trichoderma foram identificados. Embora T. reesei QM9414 tenha apresentado a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acremonium; Aspergillus; Paecilomyces; Penicillium; Trichoderma; Celulase; Endoglucanase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001100008
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Biochemical properties of an extracellular β-D-fructofuranosidase II produced by Aspergillus phoenicis under Solid-Sate Fermentation using soy bran as substrate Electron. J. Biotechnol.
Rustiguel,Cynthia Barbosa; Oliveira,Arthur Henrique Cavalcanti de; Terenzi,Héctor Francisco; Jorge,João Atílio; Guimarães,Luis Henrique Souza.
The filamentous fungus A. phoenicis produced high levels of ?-D-fructofuranosidase (FFase) when grown for 72 hrs under Solid-State Fermentation (SSF), using soy bran moistened with tap water (1:0.5 w/v) as substrate/carbon source. Two isoforms (I and II) were obtained, and FFase II was purified 18-fold to apparent homogeneity with 14% recovery. The native molecular mass of the glycoprotein (12% of carbohydrate content) was 158.5 kDa with two subunits of 85 kDa estimated by SDS-PAGE. Optima of temperature and pH were 55�C and 4.5. The enzyme was stable for more than 1 hr at 50�C and was also stable in a pH range from 7.0 to 8.0. FFase II retained 80% of activity after storage at 4�C by 200 hrs. Dichroism analysis showed the presence of random...
Tipo: Journal article Palavras-chave: Agroindustrial substrate; Aspergillus; ?-D-fructofuranosidase; Solid state fermentation; Sucrose.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200002
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Bioleaching of gold, copper and nickel from waste cellular phone PCBs and computer goldfinger motherboards by two Aspergillus nigerstrains BJM
Madrigal-Arias,Jorge Enrique; Argumedo-Delira,Rosalba; Alarcón,Alejandro; Mendoza-López,Ma. Remedios; García-Barradas,Oscar; Cruz-Sánchez,Jesús Samuel; Ferrera-Cerrato,Ronald; Jiménez-Fernández,Maribel.
In an effort to develop alternate techniques to recover metals from waste electrical and electronic equipment (WEEE), this research evaluated the bioleaching efficiency of gold (Au), copper (Cu) and nickel (Ni) by two strains of Aspergillus niger in the presence of gold-plated finger integrated circuits found in computer motherboards (GFICMs) and cellular phone printed circuit boards (PCBs). These three metals were analyzed for their commercial value and their diverse applications in the industry. Au-bioleaching ranged from 42 to 1% for Aspergillus niger strain MXPE6; with the combination of Aspergillus niger MXPE6 + Aspergillus niger MX7, the Au-bioleaching was 87 and 28% for PCBs and GFICMs, respectively. In contrast, the bioleaching of Cu by Aspergillus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioleaching; PCBs; Aspergillus; Gold; WEEE.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300707
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Characterization of a multi-tolerant tannin acyl hydrolase II from Aspergillus carbonarius produced under solid-state fermentation Electron. J. Biotechnol.
Valera,Larissa Serrani; Jorge,João Atílio; Guimarães,Luis Henrique Souza.
Background Tannases are enzymes with biotechnological potential produced mainly by microorganisms as filamentous fungi. In this context, the production and characterization of a multi-tolerant tannase from Aspergillus carbonarius is described. Results The filamentous fungus A. carbonarius produced high levels of tannase when cultivated under solid-state fermentation using green tea leaves as substrate/carbon source and tap water at a 1:1 ratio as the moisture agent for 72 h at 30°C. Two tannase activity peaks were obtained during the purification step using DEAE-Cellulose. The second peak (peak II) was purified 11-fold with 14% recovery from a Sepharose CL-6B chromatographic column. The tannase from peak II (tannase II) was characterized as a heterodimeric...
Tipo: Journal article Palavras-chave: Aspergillus; Microbial enzymes; Solid-state fermentation; Tannase; Tannic acid.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600013
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Chemical constituents of Aspergillus sp EJC08 isolated as endophyte from Bauhinia guianensis and their antimicrobial activity Anais da ABC (AABC)
Pinheiro,Eduardo A.A.; Carvalho,Josiwander M.; Santos,Diellem C.P. dos; Feitosa,André O.; Marinho,Patrícia S.B.; Guilhon,Giselle Maria S.P.; Santos,Lourivaldo S.; Souza,Afonso L.D. de; Marinho,Andrey M.R..
The present work reports the isolation of five compounds from Aspergillus sp EJC08 isolated as endophytic from Bauhinia guianensis, a tipical plant of the Amazon. The compounds ergosterol (1), ergosterol peroxide (2), mevalolactone (3), monomethylsulochrin (4) and trypacidin A (5) were isolated by chromatographic procedures and identified by spectral methods of 1D and 2D NMR and MS. Compounds 3, 4 and 5 were tested against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus and showed good activity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chemical constituents; Antimicrobial assay; Aspergillus; Bauhinia guianensis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401247
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Controle de fungos presentes no ar e em sementes de feijão durante armazenamento AGRIAMBI
Borém,Flávio M.; Resende,Osvaldo; Machado,José da C.; Fontenelle,Isa M. R.; Sousa,Frederico F. de.
Objetivou-se, no presente trabalho, avaliar a eficácia de um equipamento redutor de inóculo (ERI) na preservação da qualidade sanitária de sementes de feijão (Phaseolus vulgaris L.) durante o armazenamento em estruturas herméticas com circulação de ar forçado. A presença de inóculo no ar foi monitorada a partir da exposição de placas de Petri com meio de cultura BDA salino, contando-se o número de unidades formadoras de colônias (UFC) dos fungos Aspergillus spp. e Penicillium spp. As análises sanitárias das sementes foram realizada pelo método do papel de filtro (Blotter test), calculando-se o índice de ocorrência de Aspergillus spp. e Penicillium spp. Durante o período de armazenamento, houve um aumento na incidência de fungos nas sementes, independente...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Equipamento redutor de inóculo; Sanidade de sementes; Aspergillus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662006000300017
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Corn diseases in Thailand Thai Agricultural
Siripong Intrama; Winit Changsri; Udom Pupipat.
Palavras-chave: Corn; Maize; Plant diseases; Curvularia; Helminthosporium; Rhizopus; Physoderma; Aspergillus; Rusts; Diplodia; Penicillium; Gibberella; Pythium; Thailand; ข้าวโพด; โรคพืช; ประเทศไทย.
Ano: 1964 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/3737
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Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain PAB
Silva,André Ribeiro da; Zanin,Lívia Montanheiro Médici; Ishikawa,Angélica Tieme; Yamashita,Cassia Reika Takabayashi; Fracalossi,Felipe Pedote; Amorim,Thaís Marques; Itano,Eiko Nakagawa; Kawamaura,Osamu; Hirooka,Elisa Yoko.
Abstract: The objective of this work was to standardize and validate an indirect competitive enzyme-linked immunosorbent assay (ic-Elisa), as a low-cost tool, to monitor the presence of aflatoxin in common and blanched peanuts (Arachis hypogaea) in the production chain. The presence of aflatoxin B1, moisture content, and water activity were analyzed in 60 samples of the peanut cultivar Runner IAC 886, from the 2014/2015 and 2015/2016 harvests of the region of Alta Paulista, in the state of São Paulo, Brazil. The validation showed an adequate linearity (R2 = 0.999), and limits of detection and quantification of 1.13 and 3.59 μg kg-1, respectively. Recovery rates of 104, 102, and 107% at the concentrations of 4, 10, and 20 μg kg-1 aflatoxin B1, respectively,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus; Aflatoxin B1; Blanched peanuts; Contamination; Mycotoxin.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000300361
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Development of RFLP-PCR method for the identification of medically important Aspergillus species using single restriction enzyme MwoI BJM
Diba,K.; Mirhendi,H.; Kordbacheh,P.; Rezaie,S..
In this study we attempted to modify the PCR-RFLP method using restriction enzyme MwoI for the identification of medically important Aspergillus species. Our subjects included nine standard Aspergillus species and 205 Aspergillus isolates of approved hospital acquired infections and hospital indoor sources. First of all, Aspergillus isolates were identified in the level of species by using morphologic method. A twenty four hours culture was performed for each isolates to harvest Aspergillus mycelia and then genomic DNA was extracted using Phenol-Chloroform method. PCR-RFLP using single restriction enzyme MwoI was performed in ITS regions of rDNA gene. The electrophoresis data were analyzed and compared with those of morphologic identifications. Total of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus; Identification; MwoI.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200018
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Distribution of filamentous fungi causing invasive fungal disease at the haematological unit, Hospital de Clínicas de Porto Alegre, Brazil BJID
Aquino,Valério Rodrigues; Verçosa,Emanuelle Bergonsi; Falhauber,Gustavo; Lunardi,Luciano Werle; Silla,Lucia; Pasqualotto,Alessandro Comarú.
Very limited data are available in the literature to elucidate the aetiology of invasive mould infections in Latin America. Here we report that Aspergillus species caused only half of such cases in a cohort study conducted over 21 months in a university hospital in Porto Alegre, Southern Brazil. Fusarium spp. were the second most prevalent moulds (20.7%), followed by Zygomycetes (13.8%). The importance of obtaining local epidemiological data for adequately guiding empirical antifungal therapy is reinforced.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus; Fusarium; Mold infections; Epidemiology; Zygomycosis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300013
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Effect of cinnamomum zeylanicum blume essential oil on the rowth and morphogenesis of some potentially pathogenic Aspergillus species BJM
Carmo,Egberto Santos; Lima,Edeltrudes de Oliveira; Souza,Evandro Leite de; Sousa,Frederico Barbosa de.
Cinnamomum zeylanicum Blume is known for a wide range of medicinal properties. This study aimed to assess the interference of C. zeylanicum essential oil on the growth and morphogenesis of some potentially pathogenic Aspergillus species. The essential oil presented strong antifungal effect causing the growth inhibition of the assayed strains and development of large growth inhibition zones. MIC50 and MIC90 values were 40 and 80 µL/mL, respectively. 80, 40 and 20 µL/mL of the oil strongly inhibited the radial mycelial growth of A. niger, A. flavus and A. fumigatus along 14 days. 80 and 40 µL/mL of the oil caused a 100% inhibition of the fungal spore germination. Main morphological changes observed under light microscopy provided by the essential oil in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus; Cinnamomum zeylanicum Blume; Essential oil; Antifungal activity.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100021
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Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L. Electron. J. Biotechnol.
Rudrappa,Thimmaraju; Neelwarne,Bhagyalakshmi; Lakshmanan,Venkatachalam; Reddampalli Venkataramareddy,Sreedhar; Gokare Aswathanarayana,Ravishankar.
Genetically transformed roots of red beet produce copious levels of peroxidase (POD) - a multifunctional enzyme with a number of commercial applications. In an effort to elicit the POD activity, the cultures were treated with biotic elicitors such as dry cell powders of microbial cultures (0.1-0.5% w/v) and the respective culture filtrates (1-5% v/v). Similarly, abiotic elicitors, particularly metal ions (2-8 folds of that present in the nutrient medium), the plant hormone Thidiazuron (at 0.25-1 ppm) and other bio-molecules such as Glutathione (at 0.5-10 mM) and Methyl jasmonate (at 20-100 µM) were used. It was observed that dry cell powder of Candida versatilis significantly elicited the enzyme activity (3.52-fold higher than the control) followed by...
Tipo: Journal article Palavras-chave: Aspergillus; Calcium; Culture filtrate; Dried cell powder; Elicitor; Glutathione; Methyljasmonate; Rhizophus; Thidiazuron.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500006
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Embrapa & escola: ciência alimentando o Brasil. Infoteca-e
EMBRAPA UVA E VINHO..
bitstream/item/148700/1/Folder-Embrapa-e-Escola-2016-curva.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Tempo nos alimentos; Desequilibro ambiental; Recursos naturais; Fungos filamentosos; Análise sensorial; Sentidos humanos; Variedade Gala (maçã); Variedade Fuji (maçã); Variedade Pink Lady (maçã); Variedade Granny (maçã); Vaariedade Smith (maçã); Fungos; Microorganismo; Recursos natural; Embrapa & Escola CNPUV.; E&ECNPUV; Clima; Climatologia; Alimento; Temperatura; Frio; Mata; Recurso hídrico; Micotoxina; Maca.; Aspergillus; Penicillium..
Ano: 2016 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1054575
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